बीज की गुणवत्ता-परीक्षण के लिए जैव-मार्कर

Enzyme activity- Dehydrogenase activity- viability test through TZ test

Protein profiles- loss of protein subunits with advancement in ageing and loss of viability

100 100 37 22 0 0 % Germination %

DNA integrity-. Loss of DNA integrity and shearing of DNA with advancement in ageing and loss of viability

Note; M-marker, D-Days after accelerated ageing

F 3D 6D 9 D 12D 15D M

Genetic purity testing- Biochemical and Molecular markers

Proteins- storage proteins

Dominant markers

Staining sensitive

Compact profile –difficult to identify though markers present

F H M F H M

Isozymes- Peroxidases

Co-dominant

Non-toxic

Staining sensitive and stage specific

Markers from both the parent

F H M

DNA markers- SSR markers

Co-dominant

Reliable and stable

Markers from both the parent

Note: F- female parent H- hybrid M-male parent

Crop: Chillies